AdvanceBio Gly-X Technology
AdvanceBio Gly-X technology is a new generation of N-glycan sample preparation. Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. AdvanceBio Gly-X Increases analytical sensitivity with a variety of dye options, while increasing sample throughput.
- N-Glycan Sample Preparation Kits
This product is not available for purchase by the general public.
Product Details
Features
- Three dyes are currently available for the Gly-X platform:
- InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
- 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
- InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Superseded by InstantPC, which has higher fluorescence and MS signal (see Agilent publication 5994-0942EN). Sample prep time: ~1 hour.
Videos
Literature
- Application Notes
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Comparative Analysis of Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit
This application note compares the Agilent AdvanceBio Gly-X N-glycan prep with Instant PC kit and Waters GlycoWorks RapiFluor-MS N-glycan kit for N-glycan analysis.- Application Notes
- English
- 17 May 2023
- 782.74 KB
Agilent Biocolumns - Critical Quality Attributes - Glycan Compendium
Application compendium for glycan analysis.- Application Notes
- English
- 24 Nov 2021
- 6.73 MB
Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS
Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS- Application Notes
- English
- 23 Sep 2021
- 1.19 MB
Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans
The characterization of N-glycans is essential to the development of biotherapeutics.- Application Notes
- English
- 08 Jan 2021
- 601.12 KB
Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis
A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.- Application Notes
- English
- 17 Sep 2019
- 880.59 KB
- Article Reprints
- Brochures
- Catalogs
- Flyers
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Support
- FAQs
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AdvanceBio Gly-X Technology Frequently Asked Questions
Frequently Asked Questions for Gly-X Technology - User Manuals
Featured References
- Publications
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- View all AdvanceBio Product Citations Learn More
Related Products
News
Quick and Efficient Sample Cleanup for Biomolecule Analysis
Learn how to use centrifugation with gel filtration cartridges for sample cleanup. This approach is a rapid, convenient, straight-forward technique that can be used to easily avoid the problems associated with analysis of formulated monoclonal antibodies.- 01 Apr 2024
Professor Michael Butler, head of the National Institute for Bioprocessing Research and Training (NIBRT) Cell Technology Group (CTG), explains that the characterisation and analysis of glycans is important for the biopharma sector as it can help in the development of therapeutic agents, such as monoclonal antibodies and other recombinant proteins. These are used in therapies in a range of serious conditions including cancer, inflammatory diseases, and cardiovascular diseases. The mechanism of action depends upon their strong affinity to molecular targets associated with disease.
- Drug Discovery World (DDW)
- 10 Oct 2022
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