AdvanceBio Oligonucleotide Columns
AdvanceBio Oligonucleotide columns offer high-quality, high-resolution ion-pair reversed-phase (IP-RP) column chemistry platform that support analytical characterization through purification with seamless method scale-up. To successfully separate trityl-off, deprotected oligonucleotides, HPLC columns need to have high resolving power and be robust enough to withstand high pH and temperature, which provide better separation and denaturing conditions for optimal resolution and oligonucleotide separation.
AdvanceBio Oligonucleotide columns feature high-efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance. AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities, and biopharmaceuticals.
AdvanceBio Oligonucleotide columns feature high-efficiency, 2.7 and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance. AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization and bioanalysis of proteins, antibodies, conjugates, new biological entities, and biopharmaceuticals.
Product Details
Features
- Robust particle chemistry with high pH and temperature stability – allows for increased resolution under optimal denaturing conditions for superior peak shape and separation
- Fully scalable particle chemistry platform available in 2.7 and 4 μm Poroshell particle – simplifies method scale-up and eliminates need for chemistry re-validation when scaling up to purification
- 4 μm Poroshell particle chemistry in 21.2 mm ID preparative columns – higher efficiency and increased resolution for improved yield and purity of target product
- Increased flexibility – compatible with HPLC and UHPLC instruments via 2.7 μm Poroshell particles
- Improved reliability of results – high-resolution separations delivered by efficient Poroshell particle morphology
- Reduced costs – long column lifetimes from robust, high-pH resistant, chemically modified silica
How It Works