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Features

Modular, upgradable imaging modes, plus multimode detection enables a range of cell-based assays and applications beyond a standard microplate reader
Combination of hybrid plate reader and advanced microscopy mode increases productivity for a system that is ready for any assay
Hit-picking capability reduces time and computer memory with no unnecessary data collection or analysis
Fully automated microscopy tasks, from slide scanning to time-lapse live cell assays, and flexible hardware increase productivity and reduce manual labor
Combination of monochromator and filter optics delivers flexibility and performance to microplate assays, and choice of optics allows you to read microplates in a wide range of applications
BioTek Gen5 software enables walk-away automation for your image process and analysis workflows and data analysis tasks
Variable bandwidth quad monochromator optics enhance assay performance including increased sensitivity and lower limits of detection

Specifications


Automation Compatibility	BioSpa BioStack 3rd-party BenchCel
Camera	Sony CMOS, 16-bit color
Environment Control	4-Zone Incubation to 65 °C Condensation Control Peltier Cooling CO2, O2 Control
Filter Detection Modes	BRET Fluorescence Intensity Fluorescence Polarization Luminescence Time Resolved Fluorescence TR-FRET Alpha (Laser)
Imager/Reader Options	Joystick stage control Laser autofocus Reagent injectors
Imaging Methods	Montage Multi-color Single-color Time lapse Z-stacking
Imaging Modes	Widefield fluorescence Brightfield High contrast brightfield Color brightfield Phase contrast
Magnification Range	1.25x to 60x air 4x to 40x phase contrast
Microscope Type	Inverted microscope
Monochromator Detection Modes	UV-Vis Absorbance Fluorescence Intensity (variable bandwidth) Luminescence Time Resolved Fluorescence (secondary)
Shaking	Linear Orbital Double-orbital
Supported Labware	6 to 1536-well plates Slides Cell culture dishes Cell culture flasks (T25) Hemocytometers Chamber slides Take3/Take3 Trio

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How It Works

Applications

Technology

Peltier cooling for environmental stability

The Peltier Cooling Module promotes rapid interior cool down after incubated processes, allowing efficient switching between multiple applications without unwanted temperature changes. For sensitive assays run at room temperature, the Cooling Module maintains environmental stability within Cytation at < 1 °C change.

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Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser. Learn More

Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance. Learn More

Proprietary Laser Autofocus

Laser autofocus and image-based (or software-based) autofocus are two methods of autofocus widely used in microscopy. Learn More

Time-resolved fluorescence

Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) measurement. The only difference is the timing of the excitation / measurement process. Learn More

Alpha detection

AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios. Learn More

Absorbance

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator. Learn More

Fluorescence polarization

Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not. Learn More

TR-FRET

Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore. Learn More

Cell Counting

Accurate cell counting is a critical step across a spectrum of cell biology applications. Direct cell counting uses methods that identify and enumerate individual cells, as opposed to indirect methods that estimate population sizes using biochemical methods. Learn More

Object Tracking

With the incorporation of the object tracking module, available within Agilent BioTek Gen5 software for imaging and microscopy, this can be easily accomplished in a fully automated fashion. Learn More

Automatic Region of Interest Identification

This process enables walk-away slide scanning for four or more slides, if combined with Agilent automation offerings, and can be used with the Agilent BioTek Lionheart automated microscopes and Agilent BioTek Cytation imaging multimode readers. Learn More

Literature

Key Literature

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Application Notes

Specifications

Technical Overviews

White Papers

Support

FAQs

Microplate Readers - FAQs

Answers to frequently asked questions about microplate readers in general and Agilent BioTek multimode and absorbance readers specifically.

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Agilent Cell Analysis Publication Database

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Videos

Training & Events

Automated, Efficient Imaging of 3D Glioma Tumoroid Invasion to Screen for MT1-MMP Inhibitors in 96-Well Format

Fixed samples for fluorescence microscopy applications can take many shapes and forms, including cultured cells in microplates, multicellular spheroids, tissue on slides, and whole organisms.

Webinar/Training

Overcoming Pitfalls in Live Cell Imaging

In this webinar, we will discuss some of the common pitfalls that scientists encounter when setting up their live cell imaging assays and how to overcome them.

Webinar/Training

Maximizing Fluorescent Signal Using Variable Bandwidth Monochromators for Plate Reader-Based Assay Optimization

One of the challenges with multiplexing fluorophores in biological assays is being able to reliably distinguish the signal of one fluorophore from another.

Webinar/Training

Featured

BioTek Cytation 5 Cell Imaging Multimode Reader

Product Details

Peltier cooling for environmental stability

Hybrid plate reader: Flexibility and performance

Variable bandwidth for sensitivity and specificity

Proprietary Laser Autofocus

Time-resolved fluorescence

Alpha detection

Absorbance

Fluorescence polarization

TR-FRET

Cell Counting

Object Tracking

Automatic Region of Interest Identification

Download Literature

Microplate Readers - FAQs

Didn’t find what you need? Contact our dedicated Cell Analysis support teams.

Image Gallery

Agilent Cell Analysis Publication Database

Videos

Automated, Efficient Imaging of 3D Glioma Tumoroid Invasion to Screen for MT1-MMP Inhibitors in 96-Well Format

Fixed samples for fluorescence microscopy applications can take many shapes and forms, including cultured cells in microplates, multicellular spheroids, tissue on slides, and whole organisms.

Overcoming Pitfalls in Live Cell Imaging

In this webinar, we will discuss some of the common pitfalls that scientists encounter when setting up their live cell imaging assays and how to overcome them.

Maximizing Fluorescent Signal Using Variable Bandwidth Monochromators for Plate Reader-Based Assay Optimization

One of the challenges with multiplexing fluorophores in biological assays is being able to reliably distinguish the signal of one fluorophore from another.