MitoXpress Xtra Oxygen Consumption Assay
The Agilent MitoXpress Xtra oxygen consumption assay allows real-time measurement of extracellular oxygen consumption of whole cells or isolated mitochondria. It is a reliable method for determining respiration rates for metabolic characterization and to evaluate toxic effects of treatments on mitochondrial function in a high-throughput format.
Product Details
Features
- Applicable for use with a wide range of in vitro models, including adherent and suspension cells, permeabilized cells, isolated mitochondria or enzymes, tissues, 3D-cultures and spheroids, bacteria, yeasts, and molds
- Assess metabolic flexibility and substrate preference
- Directly evaluate mitochondrial toxicity of treatments
- Simple 'mix-and-measure' protocol allows multiparametric analysis with a range of other assay kits, such as the Agilent pH-Xtra glycolysis assay
- Reversible assay, making transient and fast changes in oxygen consumption observable
- The Agilent Data Visualization tool enables calculation of kinetic signal curves and slope values from experimentally derived fluorescence data
- Additional mineral oil and mineral oil droppers are available at request
How It Works
