Analytical Method | Preparative Method | |||
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System Description | ||||
Name of each chromatographic instrument (e.g., “1260 Infinity II Preparative LC” or “1260 Infinity II Analytical LC”)
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Minimum instrument flow rate
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Maximum instrument flow rate
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Volume from the mobile phase mixing point to the column inlet. Learn more |
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Time required for the autosampler to complete one injection cycle
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Solvent name (e.g., “Water + 0.1% formic acid”)
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Solvent name (e.g., “Acetonitrile + 0.1% formic acid”)
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Column Information | ||||
Stationary phase name (e.g., “ZORBAX SB-C18”).
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Column particle size
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Column internal diameter
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Column length
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Method flow rate
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This value exceeds the maximum flow rate. The gradient table will be calculated using the maximum flow rate. | ||||
Method injection volume
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Estimated concentration of the target compound in the sample
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Override preparative flow rate
Select “yes” to scale the analytical gradient program to a user-defined preparative flow rate; select “no” to use the preparative flow rate scaled from the analytical flow rate
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Total mass of purified target compound
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Current mass on column (mg) | ||||
Estimated column capacity (mg) | ||||
Column void volume (mL) |
Analytical Gradient | Preparative Gradient | ||||||
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Time (min) | Flow (mL/min) | %A | %B | Time (min) | Flow (mL/min) | %A | %B |
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