Hi-Plex
Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Hi-Plex HPLC columns are available in 8 μm and 10 μm particle sizes.
- Ion Exclusion/Ligand Exchange Columns
This product is not available for purchase by the general public.
Product Details
Features
- Agilent's recommended column for carbohydrate analysis
- Operates on a standard HPLC and does not require specialized equipment
- Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % acetonitrile as a modifier
- Available in 8 μm and 10 μm particle sizes
- Complies with USP L17, USP L19, and USP L34
Tools
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Applications
Literature
- Key Literature
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Single Quad LC/MS Analysis of Organic Acids Using an Agilent Hi-Plex Column
Organic acids are quantitated down to ppb levels using an Agilent Hi-Plex column and Single Quad detection.- Application Notes
- English
- 23 Dec 2016
- 644.98 KB
Analysis of Organic Acids by Ion Exclusion Chromatography and Single Quadrupole LC/MS
Demonstration of sensitivity and linearity of LC/MS single quad for analysis of organic acids using standards and real world samples.- Posters
- English
- 31 Oct 2016
- 1.71 MB
Support
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LC Column User Guides | Agilent
Find the information you need to ensure optimal performance of your column, such as flow rate, solvent compatibility, cleaning, storage, and usage conditions.LC and LC/MS Columns - USP Designations | Agilent
USP designations for LC and LC/MS columns
News
Per- and polyfluoroalkyl substances (PFAS) are a large group of anthropogenic chemicals that have been applied in industrial, commercial, and domestic products since the 1950s. Because of their toxicity, persistence, and bioaccumulation potential, PFAS have become global environmental pollutants. Besides the environment, the food chain represents another source of exposure, and the risk related to the presence of PFAS in foods has become of increased interest. Whole milk, infant formula, and ingredients used in infant formula production represent important foodstuffs that require sensitive methods with reporting limits at low parts per billion levels or lower for multiple PFAS. This article summarizes optimization experiments and validation of a complete workflow, including sample preparation and a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for determination of sixteen priority PFAS analytes listed by the U.S. Food and Drug Administration (FDA).
- 07 Jan 2022
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