Seahorse XF Media, Supplements & Calibrant
Agilent Seahorse XF assay media are formulated based on common culture media DMEM or RPMI, and are optimized for use in XF assays. Unlike typical cell growth or culture media, XF assay media are free of bicarbonate, low in buffer capacity, and contain no phenol red, which is important for achieving robust results. In addition, Seahorse XF media do not contain typical supplements such as glucose, glutamine, and pyruvate, allowing users to customize the media to the assay conditions of interest. Please read the Agilent Media Selection Guide to learn about media compatibility with Agilent Seahorse XF assays and how to prepare assay media properly.
Agilent Seahorse XF calibrant is used for hydrating and calibrating any XF cartridges and is included with a FluxPak.
Agilent Seahorse XF calibrant is used for hydrating and calibrating any XF cartridges and is included with a FluxPak.
- XF Assay Kits, Reagents & Cell Assay Media
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Product Details
Features
- Specifically formulated for Agilent Seahorse XF assays to achieve optimal results
- No glucose, glutamine, or pyruvate, allowing for customization
- Select media pre-adjusted to pH 7.4, which saves time on assay preparation and ensures consistent results across experiments
Technology
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time. Learn MoreLiterature
- Data Sheets
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XF Calibrant
XF Calibrant is optimized for XF sensor cartridge calibration, including XFe, XF, and XFp sensor cartridges.- Data Sheets
- English
- 06 Dec 2023
- 33.52 KB
Data sheet - 103575-503_Agilent Seahorse XF DMEM Medium pH 7.4_Data Sheet RevB1
This documents contains usage and formulation inforamtion for Agilent Seahorse XF DMEM medium pH 7.4, Part Number 103575-100- Data Sheets
- English
- 01 Feb 2021
- 96.80 KB
Data sheet - 103576-503_Agilent Seahorse XF RPMI Medium pH 7.4_Data Sheet RevB1
This documents contains usage and formulation inforamtion for Agilent Seahorse XF RPMI medium pH 7.4, Part Number 103576-100- Data Sheets
- English
- 01 Feb 2021
- 99.15 KB
XF Base Medium Minimal DMEM (0 mM Glucose)
Data sheet for the Minimal DMEM XF base medium.- Data Sheets
- English
- 23 Feb 2017
- 414.44 KB
Seahorse XF Base Medium (without Phenol Red)
Seahorse XF Base Medium (without Phenol Red)- Data Sheets
- English
- 23 Feb 2017
- 413.56 KB
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Tools
Agilent Seahorse XF Instruments Selection Guide
Evaluate and compare the Seahorse XF Analyzers.Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.News
The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.- 17 Jan 2022
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