Synthetic oligonucleotides (including small interfering RNA, antisense oligonucleotides, aptamers) have become fast-growing modalities in recent years. With the development of these candidates comes the increased need for robust analytical methods and easy-to-use data analysis workflows to characterize them. Commonly sought-after attributes of oligonucleotides include the mass, purity (and relative quantity of impurities present), and sequence.