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What does Seahorse XF technology measure?

Seahorse XF technology measures the flux of oxygen, the oxygen consumption rate [OCR], and the flux of protons, the extracellular acidification rate [ECAR], in the medium immediately surrounding cells in a microplate. OCR is proportional to mitochondrial respiration, while ECAR is proportional to glycolysis. Seahorse XF instruments measures these two energy pathways simultaneously, in real time, without labels or dyes. View a two-minute video on the Energy Pathways for details. ...two major energy pathways
  Energy Pathways
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