Separation and Purification Your Way: siRNA to mRNA
Agilent recognizes the diversity of the oligonucleotide landscape, offering the optimal chemistry for robust resolution, reproducibility, and long column lifetime.Agilent Oligonucleotide Separation and Purification Guide
Agilent AdvanceBio Oligonucleotide columns feature high efficiency, 2.7 µm and 4 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. This provides better separation and denaturing conditions for optimal resolution and oligonucleotide separations. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides making it ideal for LC/UV and LC/MS characterization workflows. In addition, we test every batch of AdvanceBio Oligonucleotide media with a resolution standard to ensure consistent performance.
The AdvanceBio Oligonucleotide column family offers a high efficiency, high resolution superficially porous particle chemistry that is available in 2.7 µm and 4 μm particle sizes with 100 Å pore size. It is a fully scalable column chemistry platform with dimensional offerings from analytical through 10 mm id semi-prep and 21.2 mm id prep. For high resolution separations of short synthetic oligos the AdvanceBio Oligonucleotide column chemistry is an ideal choice.
The Agilent PLRP-S polymeric media is physically and chemically robust and therefore well suited to the harsh mobile phase conditions required for IP-RP oligonucleotide purification. Available in a variety of different particles (3 μm to 30 μm) and pore sizes (100 Å all the way up to 4000 Å) with a dimensional offering from analytical through 100 mm id prep, PLRP-S is ideal for longer oligo sequences like mRNA or for larger process scale preparative purifications.
Together, these two Agilent column portfolios can uniquely support all your oligonucleotide IP-RP separation workflow needs from early-stage discovery through process scale purifications.With Agilent AdvanceBio Spin columns and plates, users can easily and quickly remove excess salts or undesired small molecules such as synthesis byproducts from their sample matrix, or exchange the sample matrix between different buffer conditions.
These gel-filtration-based spin columns are available in analytical scale and semipreparative scale columns for single samples, as well as 96-sample plates for high-throughput settings. The column bed contains the same crosslinked dextran particles in all three formats, so users can easily scale up or down in sample number or volume. Learn more863--------m.lzkeshun.com
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