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Agilent Seahorse XF Pro Analyzer |
Agilent Seahorse XF HS Mini Analyzer |
Agilent Seahorse XFe24 Analyzer |
| Plate Format |
Microplate |
Miniplate |
Microplate |
| Number of Assay Wells |
96 |
8 |
24 |
| Microchamber volume |
2.28 μL |
2.28 or 0.71 μL |
5.65 μL |
| Assay Working Volume |
150–275 µL |
150–275 μL |
500-1000 μL |
| Recommended Injection Volume |
25 µL |
25 µL |
75 µL |
| Controller |
Combination computer and touch screen display with full assay design, control, and analysis capability. |
Integrated full-color touch screen interface to design basic protocols and run imported templates. |
Combination computer and touch screen display with full assay design, control, and analysis capability. |
| Software |
Wave Pro controller software includes preloaded templates for quick experimental design, automatically calculates rates in real time, and provides exclusive cutting-edge data analysis tools (e.g., multifile analysis, dose-response curves, Z'), advanced template import feature and dose-response experiment set |
Create and run standard assays on controller and perform data analysis with Seahorse Analytics. |
Design and analyze assay templates on Controller or Wave desktop. |
| Best For |
- Phenotypic screening
- Testing many conditions at once
- Dose-response studies
- Spheroids
|
- Pairwise comparisons
- Phenotyping single samples
- Precious biomaterial
- Ex-vivo and primary cells
|
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| Key Advantages |
- High sensitivity
- Tight error in low ranges down to OCR of 13 pmol/min
- Verified instrument performance
- Advanced thermal control to reduce edge effect when used with XF Pro M plates
- Automated data QC
- IQ and AIQ certified instrument performance experimental flexibility
- Highest throughput
- Tested for hypoxia
- Support for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
|
- Easy to set up and run via streamlined interface
- Standardized protocols for XF Assays
- Support for assay temperatures 16-40°C (8–20°C lower ambient temp required)
|
- Tested for hypoxia
- Support for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
- Balances throughput and budget considerations
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