Seahorse XF Real-Time ATP Rate Assay Kit
Seahorse XF Real-Time ATP Rate Assay Kit
The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells. The real-time kinetic quantification delivers a dynamic picture of cellular bioenergetics, providing researchers with unique insights into cellular phenotype and function. This assay should be performed using XF pH-defined media. Please refer to the assay user guide to select the correct media.
Each assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF Pro, XFe96, and XFe24 Analyzers. The XFp kit is for use with the Agilent Seahorse XF HS Mini and XFp Analyzers.
Each assay kit contains sufficient materials for six full-plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF Pro, XFe96, and XFe24 Analyzers. The XFp kit is for use with the Agilent Seahorse XF HS Mini and XFp Analyzers.
- XF Assay Kits, Reagents & Cell Assay Media
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Product Details
Features
- Simultaneously measure basal ATP production rates from mitochondrial respiration and glycolysis. Total ATP production rates can also be obtained
- Assess the effect of compounds on ATP production rates
- Generate real-time kinetic data in live cells, allowing users to assess the interplay and relationship between energy metabolism and cellular functions
- Use with Agilent Seahorse XF DMEM medium, pH 7.4 (Cat. No. 103575-100) or Seahorse XF RPMI medium, pH 7.4 (Cat. No. 103576-100)
- Reconstitute kit components directly in the assay medium, removing the need for DMSO or other solvents
- Eliminate the cost associated with failed experiments since each lot is rigorously tested and validated with functional assays to deliver consistent robust results
- Upload your assay result file to Agilent Seahorse Analytics to calculate and report parameters of the XF Real-Time ATP Rate assay, share with collaborators, or export data
Technology
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time. Learn MoreHow It Works
Literature
- Application Notes
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Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates
This application note introduces a workflow that uses the XF Real-Time ATP rate assay data for identifying metabolic modulator and drug targets.- Application Notes
- English
- 30 Jan 2023
- 1.58 MB
- Flyers
- Posters
- Selection Guides
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Support
- Material Safety Data Sheets
-
Seahorse XFp Real-Time ATP Rate Assay Kit
Material Safety Data Sheet- Material Safety Data Sheets
- English
- 03 Jul 2024
- 142.73 KB
Agilent Seahorse XF Real-Time ATP Rate Assay Kit, Part Number 103592-100
Material Safety Data Sheet- Material Safety Data Sheets
- English
- 22 Apr 2022
- 148.90 KB
- User Manuals
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Tools
Agilent Seahorse XF Instruments Selection Guide
Evaluate and compare the Seahorse XF Analyzers.Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
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News
The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.- 17 Jan 2022
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