xCELLigence RTCA eSight - Imaging & Impedance
RUO
The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell biology processes using live, simultaneous, and real time biosensor impedance-based and image-based measurements. The eSight system combines the label-free xCELLigence RTCA technology with live cell imaging in 3 colors (red, green, and blue). This combination allows for informative live cell analysis and increased insight into cell health.
For Research Use Only. Not for use in diagnostic procedures.
- RTCA Analyzers
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Product Details
Features
- Combine label-free xCELLigence real time cell analysis technology with live cell imaging in red, green, and blue for increased confidence in your cell analysis and conclusions.
- Deliver physiologically relevant data with a simple workflow that adapts to differing experimental protocols while monitoring cell health, strength of adhesion, changes in morphology, proliferation, and cytolysis in primary cell cultures or standard tissue culture cell lines.
- Utilize flexible live cell imaging with brightfield capabilities, three fluorescence channels, a multitude of well plate formats, and the capability of user-defined schedules.
- Analyze a continuous readout of cell number, proliferation rate, cell size/shape, and cell-substrate attachment quality.
Technology
Single Setup for Dual Measurements
Live cell imaging and real-time biosensor measurements can be performed on the same cell populations, which provides insightful information on cell behavior. The streamlined workflow, high reproducibility, and quantitative kinetics of the eSight system makes it ideal for a wide range of cell-based assays such as proliferation, cytotoxicity, and apoptosis. Learn MoreMulti-modal Data Acquisition
In concert with the biosensor measurements, cell images can be captured in real-time, thereby providing a spatial and temporally dynamic view of cell populations, which can further validate time-dependent cell health and behavior at an unprecedented level of detail for any cell-based assay. Learn MorePatented Real Time Cell Analysis
The impedance-based xCELLigence technology utilizes proprietary microplates (E-Plates) embedded with gold biosensors at the bottom of each well, which serve to non-invasively quantify cell behavior. Over the course of an experiment, the biosensors monitor cell metrics such as proliferation, adhesion strength, changes in morphology, migration, differentiation, and much more. Learn MoreExpanded eSight Capabilities and Live-Cell Imaging Applications
Generate highly reproducible data and derive more meaningful biological insights- all inside your incubator. Investigate a wide range of cell models on the eSight. Now introducing Dilution Cloning, Scratch Wound Healing, Immunotherapy, Cell-by-Cell Analysis, 3D Spheroid Invasion and Immune Cell Killing applications. Learn MoreHow It Works
Applications
Literature
- Key Literature
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Biosensor Technology Meets Live Cell Imaging
xCELLigence RTCA eSight 4pg brochure - Biosensor Technology Meets Live Cell Imaging- Brochures
- English
- 09 Aug 2019
- 1.77 MB
Measure Cell Movement, Health, and Function With xCELLigence RTCA eSight
Multiparametric real-time automated readouts using simultaneous cellular impedance and live cell imaging.- Brochures
- English
- 05 Jun 2023
- 6.70 MB
Continuous, Label-Free Monitoring of Immune Cell Clustering and Proliferation Using Agilent xCELLigence RTCA eSight
The eSight platform provides an efficient way to dynamically monitor immune cell activation and clustering to advance tumor immunotherapy development. Here, we evaluate CAR-T and NK92 cell killing efficacy.- Application Notes
- English
- 04 Mar 2023
- 18.11 MB
- Application Notes
- Brochures
- Flyers
- Technical Overviews
Support
- Instruction Sheets
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Dell Ownership Transfer Process
Dell Ownership transferring process- Instruction Sheets
- English
- 24 Aug 2020
- 417.41 KB
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Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.Customer Testimonials
Dr. Zelia Geouveia
Institut Curie We are a laboratory working in cancer research, namely chimeric antigen receptor (CAR)-T cells targeting different antigens in the tumor. We were seeking a high throughput platform that would allow us to evaluate CAR-T cells killing in real-time. We were also interested in visualizing the killing in real time. The xCELLigence RTCA eSight was one of the technologies that could provide such information using impedance and live cell imaging (one machine, two different and precious information). When compared with other instruments that could provide imaging information only, the image quality was similar but we would not have the impedance for comparison. Thus, we decided that having both View MoreVideos
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