Seahorse XF Pro Analyzer Scientists Choice Awards 2023 Winner
Seahorse XF Pro Analyzer
Agilent Seahorse XF Pro analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in a 96-well format. This analyzer features excellent OCR precision at low rates, verified instrument performance, optimized temperature control, and is automation enabled.
OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
The XF Pro analyzer is also equipped with advanced software, standardized workflows, and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.
OCR, PER, and ATP production rates are critical measurements of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
The XF Pro analyzer is also equipped with advanced software, standardized workflows, and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.
- XF Analyzers
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Product Details
Features
- Live cell, real-time analysis of cellular energy metabolism platform in 96-well format
- Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates
- Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as na?ve T cells
- High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
- Compatible with XF Pro M plate to reduce edge effect
- Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time
- Verified instrument performance to ensure intraplate and interplate data consistency
- Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
- Advanced Pro Controller software for easy assay protocol creation with user guided interface
- Data Quality View in Wave Pro software for automated flagging of outliers
Specifications
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How It Works
Literature
- Key Literature
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Agilent Seahorse XF Pro Analyzer
Drive research and therapeutic discovery with the transformative live-cell metabolic assay platform found in the Seahorse XF Pro analyzer.- Brochures
- English
- 10 Apr 2023
- 15.40 MB
Seahorse XF Instrument Selection Guide
Instrument Selection Guide compares all XF Analyzer specifications- Selection Guides
- English
- 27 Mar 2023
- 502.19 KB
Agilent Seahorse XF Assay Journey Poster
Gain deeper insights into the critical functions that drive cellular processes with the Agilent Seahorse XF Analyzer- Posters
- English
- 12 Sep 2022
- 691.99 KB
- Application Notes
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Tools
Discover how the Agilent Seahorse XF Pro analyzer is transforming the measurement of bioenergetics in live cells.
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.Agilent Seahorse XF Instruments Selection Guide
Evaluate and compare the Seahorse XF Analyzers.Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.Solution for Normalizing Seahorse XF Data
Learn how the Seahorse XF Imaging & Normalization System delivers a robust solution for resolving XF data
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Agilent Cell Analysis Webinar Series
View informative Agilent Cell Analysis Technology webinars on a variety of research subjects.
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News
Agilent Seahorse XF Pro analyzer won the 2023 Scientists’ Choice Awards? as the best new drug discovery & development product of 2022
Agilent Seahorse XF Pro analyzer is an enhanced metabolic assay platform. It features improved precision, pharma-oriented workflows, and new software capabilities, empowering scientists to develop next generation therapeutics and ensuring drug safety.- 09 Mar 2023
The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.- 17 Jan 2022
Technology
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time. Learn More Related Products
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